Nucleotide sequence of an anti-fluorescyl hapten antibody heavy chain variable region gene from a BALB/c mouse hybridoma cell line.
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منابع مشابه
Nucleotide and translated amino acid sequences of cDNA coding for the variable regions of the light and heavy chains of mouse hybridoma antibodies to blood group A and B substances.
This is the first report of nucleotide and translated amino acid sequences of the variable region light (VL) and heavy (VH) chains of mouse monoclonal hybridoma anti-blood group A and B substances, the combining sites of which have been mapped. Monoclonal hybridoma anti-A and anti-B produced in BALB/c mice by immunization with A or B blood group substances, with A1 erythrocytes, and water-solub...
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We report here a new BALB/c mouse kappa light chain variable regk>n(Vk) genomic DNA sequence. Genbank search showed that this Vk gene has the highest homology (63%) with a human Vk gene EV15 (1). This Vk gene was cloned from the genomic DNA library of a murine hybridoma cell line designated 33.28 (2) by using a 2.7 kb Hindm fragment of mouse germline Jk region as probe (3). In hybridoma cell li...
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A cDNA clone was constructed from a mRNA encoding an anti-GAT (Glu60 Ala30 Tyr10) BALB/c monoclonal antibody heavy chain. Its sequence, covering codons -5 to 162 and therefore encompassing the complete V-D-J region, was determined. Surprisingly, the sequence of the VH gene-encoded region was almost identical with that of the BALB/c VH anti-HNP (4-hydroxy-3-nitrophenyl) acetyl VH region, suggest...
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The primary structure of A/J anti-p-azophenylarsonate (anti-Ars) antibodies expressing the major A-strain cross-reactive idiotype (CRIA) has provided important insights into issues of antibody diversity and the molecular basis of idiotypy in this important model system. Until recently, this idiotype was thought to be rarely, if ever, expressed in BALB/c mice. Indeed, it has been reported that B...
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Recently, we reported evidence for the existence of an immunoglobulin X light chain (Ax) whose variable region differs from those encoded by the known Vk gene segments Vkl and Vx2. Expression of Xx was detected in some hybridomas elicited by treatment of a BALB/c mouse with rabbit anti-X2 antibodies coupled to bacterial lipopolysaccharide [Sanchez, P. & Cazenave, P.-A. (1987) J. Exp. Med. 166, ...
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عنوان ژورنال:
- Nucleic acids research
دوره 15 15 شماره
صفحات -
تاریخ انتشار 1987